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Effect of Concentration of Substrate on Enzymes
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| Submitted: Thu Jul 11 2002
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EFFECT OF CONCENTRATION OF SUBSTRATE ON ENZYMES AIM - To find the effect of 'concentration of substrate' on an enzyme controlled reaction and HYPOTHESIS - To prove the hypothesis: as the concentration doubles so does the rate of reaction, A point is reached however, where all sites are being used and by raising the substrate concentration the rate won't increase as the limiting factor is the amount of enzymes. RESEARCH - A test that could be done is timing the speed of a reaction with obvious end results. In most instances it is hard to record the exact finish. The reactants being used are hydrogen peroxide and the Enzyme - Catalase. This reaction produces O2 and H2O. These are not easy to see being made and a way is needed to facilitate this. pH is hard to measure to see the H2O2 change to neutral. A colorimeter could be used for...
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