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Investigation of the precision and accuracy of various analytical techniques in determining the identity and quantity of barbiturates in a mixture.
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Investigation of the precision and accuracy of various analytical techniques in determining the identity and quantity of barbiturates in a mixture. Aim The aim of this investigation was to identify the barbiturates given to us in a mixture (Mixture A) and to quantify the amounts present using a variety of analytical procedures for confirmation of results. Some techniques were used for preliminary investigation. Qualitative analysis Thin layer chromatography (TLC), infra red (IR) and Raman spectroscopy were used in determining which barbiturates were present in the mixture. A melting point determination was not done however because the melting point of a mixture of compounds will be less than the melting point of the individual compounds so therefore TLC analysis An analyte migrates up a layer of stationary phase under the influence of a mobile phase, which moves through the stationary phase by capillary action. The distance moved by the analyte is determined by its relative affinity for...
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