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Experiment - the identification of milk sugar will be determined by paper chromatography - The estimation of concentration of lactose, which is milk sugar, will be determined by a titration against alkaline copper (II) sulphate
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Introduction In this experiment the identification of milk sugar will be determined by paper chromatography. The estimation of concentration of lactose, which is milk sugar, will be determined by a titration against alkaline copper (II) sulphate. This experiment will also confirm that lactose is milk sugar. The experiment will also estimate how much lactose there is in milk. The concentration of lactose will be determined by the pH of acid produced; when lactose reacts with alkaline copper (II) sulphate, an oxidation reaction occurs and copper (I) sulphate is produced along with carboxylic acid. R-CHO + Cu(II)2+ Cu(I)+ + R-COOH Lactose Copper(II) Copper(I) Carboxylic Acid Milk sugar from milk is separated using dialysed iron. Dialysed iron is used to precipitate the milk proteins. The proteins are removed using filtration, and a colourless solution is obtained. The (milk extract) protein-free filtrate; can then be titrated against Benedict's quantitative reagent (alkaline copper (II) sulphate) to estimate the reducing sugar content of milk. The...
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