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This report gives a technical background on protein engineering and its applications as well as a literature survey on the research that has been carried out in the area.
Member rating: No Rating | Words: | Submitted: Mon Jun 19 2006
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TABLE OF CONTENTS 1 SUMMARY 1 2 INTRODUCTION 2 3 GOALS OF PROTEIN ENGINEERING 3 3.1 MACROMOLECULAR RECOGNITION 3 3.2 TARGETED DRUG DELIVERY 4 3.3 BIOREMEDIATION 4 3.4 ENGINEERED BIOLOGICAL CATALYSTS 4 4 PROTEIN COMPOSITION AND STRUCTURAL COMPONENTS 4 5 PROTEIN ENGINEERING IN THE BIOTECHNOLOGY INDUSTRY 7 6 ISSUES TO CONSIDER IN PROTEIN ENGINEERING 7 6.1 ANALYTICAL TECHNIQUES 7 6.2 CHOICE OF ATTRIBUTES 8 6.3 INDUSTRIAL ISSUES 8 6.4 UNWANTED SIDE REACTIONS 8 7 METHODS IN PROTEIN ENGINEERING 9 8 SITE- DIRECTED MUTAGENESIS 9 8.1 PRINCIPLE OF SITE-DIRECTED MUTAGENESIS 10 8.2 METHODS FOR PERFORMING SITE-DIRECTED MUTAGENESIS 11 8.2.1 IN VITRO CHEMICAL MUTAGENESIS 11 8.2.2 OLIGONUCLEOTIDE-BASED MUTAGENESIS 12 8.2.3 CASSETTE MUTAGENESIS 13 8.2.4 PCR-BASED MUTAGENESIS 14 8.2.5 SATURATION MUTAGENESIS 15 9 APPLICATIONS OF SITE-DIRECTED MUTAGENESIS 16 9.1 TOLERANCE OF PROTEINS TO AMINO ACID REPLACEMENT 16 9.2 TAILORING AND DELIVERING PROTEIN TO INDUSTRY 16 9.3 MUTAGENESIS OF NONCODING SEQUENCES OF DNA 16 9.4 MAPPING FUNCTIONAL DOMAINS OF PROTEINS 16 10 LITERATURE SURVEY 17 10.1 PROTEIN ENGINEERING 17 10.2 SITE-DIRECTED MUTAGENESIS 19 11 CONCLUSION 21 1 SUMMARY This report gives a technical background on protein engineering and its applications as well as a literature survey on the research that has been carried out in the area. It then focuses on one main method of protein engineering i.e. site-directed mutagenesis. Protein...
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