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The hypothalamic hormone, growth hormone-releasing hormone (GHRH).
Member rating: No Rating | Words: | Submitted: Mon Jun 19 2006
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Introduction The hypothalamic hormone, growth hormone-releasing hormone (GHRH), stimulates the release of growth hormone (GH) from cells of the pituitary. It is known that GHRH binds to receptors on the plasma membrane of pituitary cells to activate the cAMP second messenger system leading to GH release. A novel peptide has recently been synthesized in the laboratory that also stimulates the release of GH from pituitary cells. This peptide has been named growth hormone stimulator (GHS) In this paper, we attempt to characterize the action mechanism by which the GHS affects the pituitary cells. Materials and Methods Time Course Experiment The serum-free media prepared before the experiment contained: 0.2% BST, 120 nM transferring, 100 nM hydrocortisone, 0.6 nM triiodothyronine, 5 U/litre insulin, 3 nM glucagon, 50 nM parathyroid hormone, 2 mM glutamine, 15 nM EGF, and penicillin/streptomycin. Four culture dishes containing GH4 cells were used per time point, for each of GHRH and GHS....
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