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BIOL 1104 Biological Techniques, Instrumentation and Data Processing Experiment Laboratory Report Name: mak nga ying U.no: 2003273244 Group no: 5b Date: 13-11-2003 Tittle: ion- exchange chromatography Objective: a. to know how the ion-exchange chromatography run b. to determine which concentration of the ammonium hydroxide can elute the most tyrosine, thought the gradient elution technique. Introductions: the ion-exchange chromatography can separate the protein molecule, according to their molecular change. There are two phase present, which is a mobile phase and stationary phase. The mobile phase refer to concentration gradient of the ammonium hydroxide solution. the stationary phase just refer to the ion exchangers. The ion exchangers can exchange with the ion in the sample protein solution. there are two types of exchangers: cation exchangers and anion exchangers. The group below demonstrate the process of the anionic exchange. At first, the chloride ion attaches on the A+ ion on the anionic exchangers, later after the addition of the sample ion, chloride ion is replaced by...

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