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The Effects of Varying Starch Concentration on a Solution of Amylase: Measurement of Enzymatic Rate Changes Using IKI.  

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ABSTRACT The Effects of Varying Starch Concentration on a Solution of Amylase: Measurement of Enzymatic Rate Changes Using IKI. Brooke Good Student, Functional Biology, Section 1003, Southwest Texas State University, San Marcos, TX 78666 The relationship between starch concentration and the enzymatic rate of amylase were investigated. Nine experimental assays were created all of which contained a solution of IKI, starch, saliva, and pH buffer. The nine assays were set up under four separate conditions containing a varying dilution of a 1 % starch concentration. The assays were then timed and their IKI absorbencies were noted. The assays containing the greatest percent starch concentration, 1%, had the highest enzymatic rate. As starch percentage was decreased enzymatic rate decreased. It was concluded that starch concentration has a direct effect on the enzymatic rate of amylase. The findings were consistent with experiments of the past of this nature performed by early scientist. INTRODUCTION Enzymes are perhaps one of the most important proteins of the human body. Enzymes such...

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