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Penicillin enrichment of Listeria monocytogenes pathogenicity mutants 9906035 University of Warwick, Coventry, Warwickshire Penicillin Enrichment of Listeria monocytogenes pathogenicity mutants Listeria monocytogenes is a facultative intracellular pathogen. This project aimed to produce strains that were defective for intracellular growth by mutagenising a culture with ultra violet light and nitrosoguanidine. Intracellular mutants were selected for using a method of ampicillin enrichment. Approximately 200 colonies survived the enrichment process but only four colonies were characterised. These putative mutants, as well as nine putative mutants isolated from a previous experiment, were used to infect a culture of the mouse fibroblast cell line, L2, to look for mutants that generate small plaques. Unfortunately this infection was unsuccessful, but the generation times of the putative mutants were calculated. One mutant had a slow generation time of 88.2 minutes, three had fast generation times of 43.8, 39.6 and 33.6 minutes. The rest had relatively normal generation times of 60 minutes. INTRODUCTION Listeria...

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