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Microbial Evaluation of Two Food samples (Chicken and Doubles) Aim: To identify and count different bacteria present in chicken and doubles (Salmonella, Staphylococcus, E. coli, and total aerobic bacteria).
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Laboratory Exercise # 1 Title: Microbial Evaluation of Two Food samples (Chicken and Doubles) Aim: To identify and count different bacteria present in chicken and doubles (Salmonella, Staphylococcus, E. coli, and total aerobic bacteria). Objectives: * To understand the preparation of media cultures * To identify and enumerate the growth of bacteria on different media * To identify the bacteria present in chicken and doubles Introduction The microbial evaluation of foods usually require that individually viable propagules are encouraged to multiply in liquid media or on the surface, or within the matrix, of a medium solidify with agar (Adams and Moss 2000). Different group of microorganisms will grow best on selected media. However the general purpose of a media is to provide the necessary nutrients needed for the growth of the organism(s) under study. Two common methods used to culture microorganisms are: (a) pour plate and (b) spread plate. In the pour plate method, a sample (usually 1ml), is pipette...
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