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Investigation  

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Investigate the effect of temperature on the rate of a reaction catalyzed by the enzyme trypsin. Plan Hypothesis To investigate the effect of temperature on the rate of reaction of the enzyme trypsin and from this, determine the enzymes optimum temperature. Introduction Enzymes are globular protein molecules, which are often defined as biological catalysts. They catalyse the breakdown of a substrate into two or more product molecules, using the cleft in its surface called an active site. The substrate molecule has a complementary shape. The random movement of the enzyme and substrate brings the substrate into the active site. An enzyme-substrate complex is temporarily formed. (1) This is because the R groups of the amino acids in the active site interact with the substrate and this interaction breaks the substrates apart. The two product molecules then depart the active site, leaving the enzyme molecule unchanged and ready to bind with another substrate molecule. Trypsin is a...

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