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Title: Temperature on Enzyme activity. Date: 29 September 2004 Aim: To find out the effect of temperature on enzyme activity. Theory: Like all enzymes, amylase catalyses a (bio) chemical reaction. A fundamental of chemical thermodynamics is that all reaction rates will increase as the temperature increases. With a typical amylase, therefore as you increase the temperature to the optimum, the rate at which an enzyme catalyses the breakdown of starch increases. Effect of temperature on reaction rate Increasing temperature will increase the rate of chemical reactions generally (kinetic energy of reactants is raised which increases the chances of favorable collisions). Enzyme catalyzed reactions will also increase with temperature due to the same reasons. However, catalytic activity depends on the enzyme having the correct conformation (molecular shape). As temperature increases the conformation of the enzyme is disrupted due to the increased kinetic energy of the enzyme molecule itself. Materials: 1. test tube rack 2. teat pipette 3. starch solution 4....

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