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Determine the amount of gas evolved by the fermentation of different sugars by yeast.
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| Submitted: Sat Oct 16 2004
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Title: Specificity of enzymes: sugars metabolized by yeast Date: 6 October, 2004 Aim: To determine the amount of gas evolved by the fermentation of different sugars by yeast. Theory: In the absence of oxygen cellular respiration stops with glycolysis. The Krebs cycle and electron transport chain do not function. Pyruvate accumulates in the cytoplasm and oxidized form of NADH (NAD+) is depleted. When all of the NAD+ is gone, glycolysis stops and the cell dies. To avoid death, cells possess alternate pathways to regenerate NAD+ and keep glycolysis functioning in the absence of oxygen. This process is called anaerobic respiration or fermentation. In anaerobes, glycolysis and fermentation are the only respiratory pathways present. In plants and some fungi, fermentation converts pyruvate (3 carbons) to ethanol (2 carbons) and CO2. Synthesis of the ethanol provides an electron (and hydrogen) acceptor that regenerates NAD+ from NADH and allows glycolysis to continue. Yeast belongs to the...
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