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We are going to compare the fragments of viral DNA by adding three different enzymes.
Member rating: No Rating | Words: | Submitted: Mon Sep 08 2003
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DNA finger printing Aim: We are going to compare the fragments of viral DNA by adding three different enzymes. Hypothesis: The fragments that have more base pairs are the ones that are going to be closer to the wells and the control is going to have only one mark. This is because the more base pairs the fragment has the harder of it to move from one side of the gel to the other and because there is no enzyme on the well with the control the DNA is not broken down, this will look as one line. Apparatus: * Microsyringe * Distilled water * Dry lambda DNA * Small tube * EcoRI enzyme * BamHI enzyme * HindI enzyme * Water bath * Agarose gel * Transparent plastic tank * Comb * Loading dye * Electrodes * Two 9V batteries Method: Used plastic gloves cleaned with alcohol during the howled experiment.> Add 100 ul of distilled water to the Lambda DNA tube. Use the 10-ul graduation on the tip 10...
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