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Uses of DNA profiling with PCR and Electrophoresis


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Uses of DNA profiling with PCR and Electrophoresis

... USES OF DNA PROFILING WITH ELECTROPHORESIS AND PCR Genetic fingerprinting, DNA testing, DNA typing, and DNA profiling are techniques used to distinguish between individuals of the same species using only samples of their DNA. Statistically, no two people will have the same number of STR (short tandem repeats). When the DNA profile of a child is compared to the profiles of its genetic parents, it is possible to match one line in each STR area with a line in that area of the mother's profile. In this way, DNA profiling can also reveal non paternity. A technique called electrophoresis is used to obtain DNA profiles, relying on sections of our DNA that are known as non-coding DNA (DNA that does not code for a protein). Electrophoresis sorts DNA strands according to length. The electrophoresis "gel" acts as a filter that sorts DNA strands. (1) The gel is created using powdered agarose, buffer, a microwave,

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