The Principles and Methodology of 2D Electrophoresis and its Application in Proteomics and Disease Diagnosis.

... The Principles and Methodology of 2D Electrophoresis and its Application in Proteomics and Disease Diagnosis. Barry Hollinshead. Biomedical Sciences Bsc, 3rd yr. CH0588 Analytical & Immunological Methods. Introduction. One of the most widely utilised analytical tools in Molecular Biology, Proteomics and disease diagnosis is the procedure and methods of electrophoresis. The principle of this procedure is that "electrophoresis is the migration of electrically charged particles or ions in solutions due to an applied electric field"1. The ability to separate substances which are almost identical including different proteins for analysis has increased throughout the past 7 decades. This is contributed to the introduction of zone electrophoresis in paper and more recently in gels like polyacrylamide and agarose. Today the methods used in electrophoresis have increased our advances in biochemistry, molecular biology studies and diagnosis of disease, for example cancer. It has also become an invaluable tool in forensic science as it can identify species and individuals1.

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