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The Lambda Protocol
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- Words:
- 1598
- Submitted:
- Thu Jul 11 2002
... The Lambda Protocol Aim: This experiment is designed to produce ?-DNA bound in gel to fragment according to the restriction enzyme used to cut it up and to the sizes of these subsequent fragments. The aim of this experiment is to produce a ?-DNA fragmentation, which will be of known sizes because the entirety of the ?-DNA genome has been mapped. So running ?-DNA alongside other DNA will allow the size of the other DNA to be found. Prediction: As in the experiment there are restriction enzymes being used on the ?-DNA, and then running it in gel electrophoresis, to separate the varying size ?-DNA fragments, then it is possible to estimate the results. The DNA, after gel electrophoresis will form bands and smears. The smears are where there are many fragments of ?-DNA of a wide range of sizes, and bands are numerous DNA fragments of similar size. Subsequently, as the entirety of
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