Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE).

... LAB REPORT Name-Surname:Pelin Yilmaz Student ID:1370790 Date of experiment:13-10-2003 Submitted to: Hatice Özel Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Purpose This technique is used for observation of proteins. It separates the molecules on the basis of their molecular charge. It can be used to examine the purity of proteins. For example we are trying to isolate some specific protein from a cell. After some processes we can find out if we have obtained the protein or not by SDS PAGE technique. Also it can be used to determine the molecular weight of proteins. The logarithm of the molecular weight of a protein is inversely proportional to its distance from the well. By simply drawing a graph by the values and writing an equation, molecular weight of every protein can be calculated from its distance to the well. Observations Calculations Molecular weight Log of Molecular Weight Distance Lactalbumin 14.2 1,1522883443830 5,40 Carbonic anhydrase 29 1,4623979978990 3,70 Albumin egg 45 1,6532125137750 2,80 Bovine albumin 66 1,8195439355420 1,80 The distance of purified protein=3.9 cm Molecular weight of purified protein= -0.1872*3.9 + 2.1632 =

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