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Separation of serum proteins and enzymes bypolyacrylamide gelelectrophoresis (PAGE)
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Separation of serum proteins and enzymes by polyacrylamide gel electrophoresis (PAGE) Contents: Page(s) Introduction and Aim 3 - 5 Method 6 Diagram of Electrophoresis Tank 7 Results 8 - 9 Discussion 10 - 11 References 12 INTRODUCTION In an average healthy, lean human body the most abundant organic compounds are proteins consisting of over half of the cellular dry weight. A protein is a polymer of its constituent amino acids which determine its structure and chemical properties, having molecular weights ranging from approximately 10.000 to one million. A protein can have many biochemical functions including transport, contraction, catalysis, metabolic regulation, structure and protection. The basic structure of an amino acid includes a carboxyl group, a hydrogen atom and an R-group (or side chain) attached to the ?-carbon (see figure 1.) Fig 1. Basic structure of amino acid It is this R-group which is used to classify an amino acid due to their tendency to interact with water at pH 7.0. These ionisable R-groups on the protein...
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