Separating biological macromolecules by Agarose gel electrophoresis.

... LAB REPORT Name-Surname:Pelin Yilmaz Student ID:1370790 Date of experiment:06-10-2003 Separating Biological Macromolecules by Agarose Gel Electrophoresis Purpose Electrophoresis is a method of separating biological macromolecules like DNA and proteins from a mixture of similar molecules. An electric current is passed through a medium containing the mixture and each kind of molecule travels through the medium at a different rate, depending on its electrical charge and size. Agarose and acrylamide gels are the media commonly used for electrophoresis. In this experiment, we used agarose gel to act as a molecular sieve. Theory There are many types of electrophoresis. I have listed 10 of them with their description below. SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis): SDS is one of the most common types of electrophoresis. SDS is a detergent, which denatures proteins by binding to the hydrophobic regions and essentially coating the linear protein sequence with a set of SDS molecules. The SDS is negatively charged and thus becomes the

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