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Role of Enzymes in DNA Replication.
Member rating: No Rating | Words: | Submitted: Sun Aug 17 2003
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Role of Enzymes in DNA Replication Sang-mun Lee L6AA (L65) DNA replcation occurs in a 5' to 3' direction. Analysis of the primary structure of DNA polymerase ? B subunit defined similarities to B subunits of eukaryotic DNA polymerases ?, ? and ? as well as the small subunits of DNA polymerase DI of Euryarchaeota. Multiple sequence alignment of these proteins revealed the presence of 12 conserved motifs and defined a novel protein superfamily. The members of the B subunit family share a common domain architecture, suggesting a similar fold, and arguing for a conserved function among these proteins. The contribution of human DNA polymerase ? to nuclear DNA replication was studied using the antibody K18 that specifically inhibits the activity of this enzyme in vitro. This antibody significantly inhibited DNA synthesis both when microinjected into nuclei of exponentially growing human fibroblasts and in isolated HeLa cell nuclei, but did not...
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