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Our aim is to cut DNA from the bacteriophage lambda into fragments using restriction enzymes from different bacteria. We also aim to separate the DNA into bands by electrophoresis and at the end observe the results.
Member rating: No Rating | Words: | Submitted: Thu Sep 04 2003
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Klaudia Stadnicka Due 30. May. 2002 Biology; IB5 LAB REPORT #7 DNA TESTING PRACTICAL - GEL ELECRTOPHORESIS AIM: Our aim is to cut DNA from the bacteriophage lambda into fragments using restriction enzymes from different bacteria. We also aim to separate the DNA into bands by electrophoresis and at the end observe the results. Hypothesis: I will be able to observe that the DNA after intact isolation and treatment with various restriction enzymes to generate pieces small enough to resolve by electrophoresis in aragose is going to separate its fragments of different sizes. The smaller fragments are going to move more quickly through the gel and the larger more slowly. The pieces of DNA are separated by size. As in the 4th well we put the uncut DNA we expect it to move the least and very little overall. Variables: The only variables I include are diverse restriction enzymes or none at all used to cut the DNA....
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