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Objectives:To amplify a 500bp fragment of lambda DNA. To understand the principles and the application of Polymerase Chain Reaction. To analyses the PCR by using Agarose Gel Electrophoresis.
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Experiment 5 and 6 Title: Polymerase Chain Reaction and analysis of Polymerase Chain Reaction sample. Objectives: 1. To amplify a 500bp fragment of lambda DNA. 2. To understand the principles and the application of Polymerase Chain Reaction. 3. To analyses the PCR by using Agarose Gel Electrophoresis. Introduction: Polymerase Chain Reaction (PCR) is a molecular biology technique that invented by Kary R. Mullis in 1985. The PCR is a technique used to replicate a fragment of DNA so as to produce many copies of a particular DNA sequence. PCR is commonly employed as an alternative to "gene cloning as a mean of amplifying genetic material for gene sequencing, and is also used to measure gene expression. The PCR only used a small amount of DNA molecule to amplify. However, it is an in vitro technique. A PCR mixture consists of four key components- two primers(about 20 nucleotides long), a target DNA sequence(about 100 to 5000 base pair),...
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