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6.54.2.10 INDUSTRIAL USE OF ENZYMES Matti Leisola, Jouni Jokela, Ossi Pastinen, Ossi Turunen Laboratory of Bioprocess Engineering, Helsinki University of Technology, Finland, and Hans Schoemaker, DSM Research, MD Geleen, The Netherlands Keywords: Industrial enzymes, speciality enzymes, protein engineering, enzyme technology, enzyme production, biocatalysis, fine chemicals Contents 1. Historical background 2. Enzyme classification 3. Enzyme production 3.1. Microbial production strains 3.2. Enzyme production by microbial fermentation 4. Protein engineering 5. Enzyme technology 6. Large scale enzyme applications 6.1. Detergents 6.2. Starch 6.3. Drinks 6.4. Textiles 6.5. Animal feed 6.6. Baking 6.7. Pulp and paper 6.8. Leather 7. Speciality enzymes 7.1. Enzymes in analytics 7.2. Enzymes in personal care products 7.3. Enzymes in DNA-technology 8. Enzymes in fine chemical production 8.1. Chirally pure amino acids and aspartame 8.2. Rare sugars 8.3. Semi synthetic penicillins 8.4. Lipase based reactions 8.5. Asymmetric synthesis 8.6. Enzymatic oligosaccharide synthesis 9. Future trends in industrial enzymology Glossary Alkaline phosphatase: An enzyme that degrades ester bonds in alkaline conditions. Amino acid amidase: An enzyme that is used in manufacturing optically pure amino acids. It hydrolyses an amide bond in natural amino acid amides. Amylase: A...

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