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Forensic Genetics
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- Sun Feb 15 2009
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... FZ3011 Forensic Genetics SECTION A Short Tandem Repeat Polymorphisms Structure STRs, also known as microsatellites or simple sequence repeats (SSR), are 2-6bp DNA sequences tandemly repeated units flanked by unique sequence. They are usually between 100-300 bp long. They are classified based on the structure of the number of core repeats (base pairs): * Dinucleotide - 2 * Trinucleotide - 3 * Tetranucleotide - 4 * Pentanucleotide - 5 (Russell, 2006). They are also divided into categories depending on the type of repeats: * Simple - contain the exact same sequence and length with non-consensus alleles. * Compound - contain repeats of two or more adjacent simple repeats (non-consensus). * Complex - can be of several repeat blocks, varying sequences or intervening sequences. * Hypervariable - complex repeats with numerous non-consensus alleles, e.g. STR loci SE33 or ACTBP2. * Microvariants - contain alleles with incomplete repeat units, e.g. allele 9.3 at TH01 locus (Butler, 2005). Occurrence They are scattered throughout the genome and
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