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DNA GEL ELECTROPHORESIS OF AN UNKNOWN SAMPLE
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SCIENTIFIC SKILLS: DNA GEL ELECTROPHORESIS OF AN UNKNOWN SAMPLE. ABSTRACT. DNA samples in a 1.2% agarose gel, which an electrical field is applied. Makes the fragments of DNA to move through the gel mesh. The rate at which allows the genotypic analysis of DNA, by the use of restriction enzymes and gel electrophosis. It works by using a DNA, which is stored at 37oC with restriction endonuclease, so that it will remain intact, when the electric field is applied. . Fragments move through the gel depending on their size, small fragments of DNA move through the gel faster then larger ones. Causing formation of the distinctive bands, caused by the electrophoresis process. The fragments migrate to the opposite side of the gel, they were originally placed. They do this, because the DNA fragments have a negative charge, and they are attracted to the opposite positive end of the gel, at the same...
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