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Briefly outline the principles of five methods of protein purification.
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Briefly outline the principles of five methods of protein purification. Techniques of protein purification have improved greatly over the last generation. This in turn has aided our understanding of proteins and their structure and function because our better protein samples have resulted in the ability to perform more conclusive experiments. Usually protein extraction and purification is only the start of perhaps determination of the protein's structure by crystallography which could lead on to designing effective inhibitors for medical purposes. Therefore purification is often an essential factor in the success of an experiment. If you want to actually obtain a sample of protein, there are several techniques that can be used. However some are clearly better for just detection of the protein's presence and it is less easy to get a sample from the results of the assay. Paper chromatography is a good example of this as ninhydrin spray and other stains...
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