Work shope on plant Cryopreservation

... Name- Venkatesh Kolluru Student number- 0803568 Course- MSc. BIOTECHNOLOGY Module- BI1102A Title of the work shop- PLANT CRYOPRESERVATION Workshop tutor- Irene Tierney AIM: To learn about the practical aspects of plant cryopreservation and its current developments in the field of plant biotechnology. INTRODUCTION: Cryopreservation is a process where the cells or the whole tissues are preserved by cooling the temperature to -196C (BP of liquid nitrogen). In these extreme conditions the biological activity even the biochemical reactions which would lead to cell death is effectively stopped. But this technique consists of problems like damage to the cell due to solution effect (used in the solution), dehydration and intra cellular ice formation. At this temperature the salts present in the cell may become crystal which can ultimately lead to fatal conditions. To overcome this problem vitrification solutions (sucrose, DMSO i.e. Di-Methyl sulphoxide, glycerol, etc.) are used. Vitrification solutions prevent the formation of ice crystals. They do it

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