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Determination of Absorbance Spectra of Photosynthetic Pigments
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Determination of Absorbance Spectra of Photosynthetic Pigments Abstract This experiment tested the hypothesis that the absorbance of light by chlorophyll a would not be equal to the absorbance by chlorophyll b at a wavelength of 480nm. Following extraction of lipid soluble pigments from silver beet by acetone and petroleum ether and separation via chromatography, the light absorbance of the individual pigments at various wavelengths was determined by spectrophotometry. Light absorption by chlorophyll a was observed to peak at 420nm and 660nm while that of chlorophyll b peaked at 440 nm and 600nm. Mean values for class results indicated that chlorophyll b (0.09) absorbed twice the amount of photons as did chlorophyll a (0.03) at a wavelength of 480nm. Since the results of t-testing gave a value (1.359) lower than the critical t- value (2.447), the results suggest that absorption values for chlorophyll a and b at 480nm were not significantly different, thus...
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