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Demonstrate the separation of plant pigments using chromatography and the rate of photosynthesis in isolated chloroplasts.
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Lab Four: Plant Pigments and Photosynthesis II. Introduction: This experiment will demonstrate the separation of plant pigments using chromatography and the rate of photosynthesis in isolated chloroplasts. Chromatography is used to separate and identify plant pigments. The solvent moves up the chromatography paper by capillary action. As the solvent moves up the paper, it carries solute molecules with it. The pigments are carried along at different rates because they are not all equally soluble in the solvent. Photosynthesis is the reaction that takes place in plants that uses carbon dioxide, water, and light to form glucose and oxygen. There are two parts to photosynthesis: the light and dark cycles. The light cycle is the conversion of solar energy to chemical energy. The solar energy drives a transfer of electrons and hydrogen from water to NADP+, forming NADPH. Water is split in the process and thus, oxygen is given off. The light...
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