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Effects of Temperature on Enzymes


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Effects of Temperature on Enzymes

... Effect of temperature on enzymes DESIGN Aspect 1 - Defining the problem Investigate the effect of temperature on enzymes, by calculating the amount of gas produced in the reaction at different temperatures. Variables * Independent variable: temperature * Dependant variable: amount of gas produced * Variables not changed: amount of potato, pH, volume of H2O2, same time, type of potato and preparation of specimen. Aspect 2 - Controlling variables 1. Collect necessary equipment and materials. 2. Set up apparatus for the experiment as shown. 3. Weigh 2g of minced potato using a scale up 1/100 of a gram. 4. Put the potato in the conical flask and add 5cm3 of buffer solution at 70°C. Swirl gently to mix. Temperature must be stable while reaction is taking place. 5. Replace the bung in the flask, ensuring that it is at right seal. 6. Fill the burette with water and introduce into it the tube that comes from the flask bung. Invert carefully

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