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Testing the Performance of an Ion Chromatography Column
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Testing the Performance of an Ion Chromatography Column Kristin Quinn March 10, 2005 Quinn 1 Kristin Quinn Instrumental Analysis Lab 10 March 2005 Ion Chromatography Introduction Ion column chromatography is used to separate both cations and anions. The separation of ions is performed by using an ion exchange resin (this lab uses an anion exchange resin which is a high molecular weight polymer). The amount of time required for certain ions to move through the column depends on the affinities of the ions. The mobile phase's anions, sodium bicarbonate in this lab, will force the ion that's being tested out of the column. Procedure 1. Given the choice of six 1000 ppm sodium salts, choose three. The solutions to choose from are: sodium fluoride, sodium chloride, sodium bromide, sodium sulfate, sodium phosphate, and sodium nitrate. 2. Prepare three 25 mL solutions each with one of the chosen sodium salts, diluting with the carbonate buffer. 3. Run an IC on each...
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