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Liquid chromatography is a technique used to separate components of a mixture to isolate them for further use in synthesis (preparative chromatography) and for identification.
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INTRODUCTION Liquid chromatography is a technique used to separate components of a mixture to isolate them for further use in synthesis (preparative chromatography) and for identification. The separation is achieved by forcing the mixture over an immobilised chemical system in a column by means of a liquid solvent stream. The individual solutes in the mixture partition differently between the moving and immobilised phases due to different chemical interactions, and travel at different rates down the column. By the time the mixture exits the column, the solutes are spatially separated and can be collected and analysed. The are two modes of chromatography: normal-phase and reverse phase chromatography. In normal-phase chromatography, the retention is governed by the interaction of the polar parts of the stationary phase and the solute. For retention to occur in normal phase, the packing must be more polar than the mobile phase with respect to the sample. Therefore,...
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