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"AN INVESTIGATION TO FIND OUT THE OPTIMUM TEMPERATURE FOR THE ACTIVITY OF LIPASE" * AIM To investigate the effect of temperature has on enzyme activity and the hypothesis that there is an optimum temperature for the activity of the enzyme lipase. * INTRODUCTION Enzymes are catalysts made up are protein molecules, usually with a globular structure. They accelerate chemical reactions in organisms that would otherwise occur to slow. (Green, Stout, Taylor, 1991) Lipase is a digestive pancreatic enzyme which works to hydrolyses fats into glycerol and fatty acids. (Simpkins, Williams, 1987) These products will react with the Sodium Carbonate (put into the mixture to raise the pH) and in effect lower the pH level towards a more acidic content. This pH change can be observed by using the indicator Phenolphthalein, which changes from a pink colour to colourless at a pH below 8.3. * HYPOTHESIS Lipase is an enzyme found in the human body and from knowledge of...

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