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With increasing concentration of urease, the time it takes to neutralize the ethanoic acid will decrease.
Member rating: No Rating | Words: | Submitted: Mon Jan 16 2006
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A study of urease Aim: To study the effect of varying concentrations of urease on the decomposition of urea. Hypothesis: With increasing concentration of urease, the time it takes to neutralize the ethanoic acid will decrease. Justification of hypothesis: The concentration of substrate molecules, in this case urea, stays constant. As enzyme concentration increases more collisions occur with the substrate molecules and so more reactions occur. As more reactions occur, the concentration of ammonia increases and the faster will the ethanoic acid be neutralized. Variables: Independent variable: The concentration of urease in the solution Dependent variable: Rate of action of urease Controlled variables: Concentration of urea in the solution, concentration of ethanoic acid in the solution, temperature of the solutions, kind of indicator used. Data collection: Total volume of solution in test tube: 9.0 mL ± 0.1 mL Concentration of ethanoic acid added to test tube: 1.0 M Volume of ethanoic acid added to test tube: 2 mL ± 0.1mL Concentration of...
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