Logged out
Access All our Essays in an Instant
Swap your work for FREE access, or pay £4.99 for instant access
What factors affect the activity of urease? What are the kinetics of the urea-urease reaction?
Member rating: No Rating | Words: | Submitted: Tue Oct 25 2005
Page Preview
On the left is an image preview of every page of this document, and below are the first 150 words with formatting removed:
Chemistry Coursework What factors affect the activity of urease? What are the kinetics of the urea-urease reaction? Aim: To investigate closely the factors that can affect the activity of urease. The effect of concentration and temperature can be studied over a period of time leading to the order and activation enthalpy of the reaction. Introduction: UREASE (NH2)2CO + 3H2O ----------> 2NH3 (aq) + CO2 (g) Urease is an enzyme found in microorganisms, invertebrates, and higher plants. It catalyses the hydrolysis of urea to ammonia and carbon dioxide. In some bacteria and invertebrates this reaction is the final step in the breakdown of complex nitrogenous compounds before ammonia is excreted. Method: Quench with known volume and concentration of HCl to stop the reaction. Some of the acid will react with the ammonia produced by Urease to determine the amount of HCl used up. Contents of the flask are titrated with standard Sodium Carbonate solution using methyl orange indicator. From...
Get instant access
- Instant, unlimited access to our documents in full
- Swap your work for free access, or pay £4.99
- To see the full version of this document and 151,744 others
