To investigate the effect of substrate concentration on the activity of an enzyme.
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| Submitted: Thu Jul 11 2002
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Biology Investigation: Enzymology- Catalase Aim: To investigate the effect of substrate concentration on the activity of an enzyme. Background Information: ( The image above shows a 3D structure of catalase from E.coli. The structure was solved using Xray Crystallography. Enzymes are biological catalysts which speed up biochemical reactions, they lower the activation energy. Enzymes are globular proteins which catalyse metabolic reactions. In an enzyme- catalysed reactions, the substrate binds to the active site of the enzyme. The enzyme and the substrate are held together in an enzyme-substrate complex by hydrophobic bonds, hydrogen bonds and ionic bonds. The enzyme then converts the substrate to the reaction products which are released leaving the enzyme to form another enzyme-substrate complex. The enzyme is recycled again and again. One enzyme molecule can carry out 1000 of reaction cycles every minute. Eventually the enzyme will breakdown. Each enzyme is unique for a certain reaction because its amino acid sequence is arranged...

