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To investigate how enzyme concentration can affect the rate of reaction, in this case the breakdown of milk protein by trypsin, a protease enzyme.
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Biology - Activity 2.10 Enzyme concentrations and enzyme activity Aim To investigate how enzyme concentration can affect the rate of reaction, in this case the breakdown of milk protein by trypsin, a protease enzyme. Hypothesis 1. The higher the concentration of trypsin, the higher the rate of breakdown of milk protein. Reason: with higher concentration of enzyme, there are more active sites available for the substrate molecules to bind with. When there are more enzymes, the collision between enzymes and substrates becomes more frequent, increasing the chance of forming enzyme-substrate complex. 2. Increase in rate of reaction is directly proportional to increase in trypsin concentration. Reason: enzyme concentration exerts a direct effect on the rate of reaction. Apparatus o Milk o Trypsin o 3 Syringes o 5 test tubes o test tube rack o 5 stopwatches o Distilled water Procedure In this experiment, the rate of reaction is determined by the time taken for a given quantity of substrate to be used up: 1. Prepare...
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