The effect of temperature on amylase activity.
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Biology coursework: effect temperature on enzyme activity Aim: I have been asked to investigate the effect of temperature on amylase activity. Prediction: I think that as the temperature is increased, the reaction rate will be increased. This is because as the temperature rises the molecules will move faster and have an increased chance of collision and the rate of reaction will be increased. If I was using human amylase from the mouth, I would predict that it would work best at around 37°C because this is the average temperature of the human body and the temperature the enzyme would work best at. However, as the Amylase I am using is from a laboratory and it is stronger, I think it will work best at around 40°C or higher. After this at around 60°C-80°C, the rate will start to decrease as the enzymes become denatured. Method: 1. Fill spotting tile with 3 drops of...

