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The aim of this experiment is to find out the effect of bile salt concentration on the rate of lipase reactions by changing just one varialble: the concentration on bile salts in the reaction.
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Robynne Shipley 10G Factors Effecting Action of Lipase Effect of Bile Salts Concentration on the Rate of Lipase Reactions Aim The aim of this experiment is to find out the effect of bile salt concentration on the rate of lipase reactions by changing just one varialble: the concentration on bile salts in the reaction. Method * Six test tubes were taken and put into a test tube rack. * Each test tube was labelled ( 3%; 2.4%; 1.8%; 1.2%; 0.6%; 0%) with the concentration of bile salts that were going to be put into them. * 5 cm³ of 3.6% fat milk was put into each of the test tubes. * 7 cm³ of sodium carbonate was then put into the test tubes. * Then the specific concentration of bile salts was added and then a few drops of the indicator phenothalin (pink in alkaline; colourless in "acidic") * Finally 1 cm³ of 5% concentration lipase was added and the...
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