The action of catalase on hydrogen peroxide.
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Biology Assessed Practical - Rosalind Brock Spring 2002 The action of catalase on hydrogen peroxide Aim The aim of this experiment is to discover the relationship between the concentration of the substrate and the rate of the reaction catalysed by an enzyme, by looking at the decomposition of hydrogen peroxide under the action of catalase, and to determine a value for Vmax and the Michaelis constant for catalase. Background Theory An enzyme is a protein biological catalyst. Catalysts speed up or slow down the rate at which chemical reactions occur. They are not used up in the reactions and can be retrieved unchanged afterwards. Biological catalysts control the rate of reactions in living things. Each enzyme is substrate specific - it can control only one reaction. For example, the digestion of starch is begun in the mouth by the enzyme amylase. An equation for this reaction can be shown like this: Amylase Starch ????? Simple...

