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The Effects of pH, Temperature, Substrate Concentration, and Enzyme Concentration on Reaction Rates Abstract A class of proteins called enzymes catalyzes almost every chemical reaction in a cell. Enzymes increase the rates of reaction for those reactions, which are already energetically favorable, by lowering the activation energy. Quite often, the rate of an enzymatically catalyzed reaction is 106-1010 times that of an uncatalyzed reaction under similar conditions making it catalyzed reaction very favorable since it will essentially save time. The purpose of this experiment is to measure the rates of reaction of the enzyme peroxidase with the substrate guaiacol under varying conditions. To examine the effect of temperature on the reaction rate, the pH, substrate concentration, and enzyme concentration are held constant and reactions are carried out at different temperatures. Each enzyme has an optimal set of conditions at which the maximum reaction rate occurs. By carrying out the four separate...

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