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The Effects of Substrate Concentration on the Activity of Catalase Preliminary work: Prediction: As the concentration of hydrogen peroxide increases then the rate of reaction (1/t) will increase. Biological Theory: Catalase behaves as a catalyst for the conversion of hydrogen peroxide into water and oxygen and is able to speed up the reaction because the shape of its active site matches the shape of the hydrogen peroxide molecule. Below is a diagram showing the lock and key mechanism through which catalase works: As the concentration of hydrogen peroxide increases, there is more substrate to fit into the active sites of the enzyme, catalase. Also, the chances of the substrate entering an active site will increase. If the amount of enzyme remains constant, the rate of reaction will increase up to a point. The rate of reaction increases until all of the active sites of the enzyme molecule are being used. If excess substrate is added, the...

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