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The Effect of Substrate Concentration on Enzyme Activity
Member rating: No Rating | Words: | Submitted: Thu Jul 11 2002
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The Effect of Substrate Concentration on Enzyme Activity Aim: To find out what effects the concentration of the substrate has on the reaction rate of the enzyme catalase. Hypothesis: The rate of the reaction will increase as the concentration of hydrogen peroxide increases until a point where the reaction rate will remain constant. Method The following equipment will be used: -side arm conical flask with delivery tube -bung -measuring cylinders (25cm3 and 100cm3) -water trough -beehive shelf -hydrogen peroxide (6%) -knife -white tile -cork borer -clamp stand -stop clock Use the cork borer to cut 10cm lengths of potato. Cut these into ten lengths of 1cm each. Put these into the conical flask. Measure out 25cm3 of the hydrogen peroxide using the smaller measuring cylinder. Add this to 25cm3 of water. Put this into the conical flask. Replace the bung immediately and start the stop clock. Record the amount of oxygen in the large measuring cylinder every 30 seconds for 5 minutes, swirling the flask continuously. Repeat this twice to produce three...
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