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The Effect of Enzyme and Substrate Concentrations On the Rate of a Reaction
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| Submitted: Thu Jul 11 2002
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The effect of enzyme and substrate concentrations on the rate of a reaction Requirements: CARE: Eye protection must be worn. * burette (50cm ) * trough or bowl * boiling tube with bung and delivery tube * graduated pipette (5cm ) and safety filler * measuring cylinder (10cm ) * hydrogen peroxide solution, 5 vol. (25cm ) * yeast suspension (20cm ), made from 2g dried yeast in 160cm water aerated for several hours * stopwatch Introduction: In this experiment I will follow the progress of a catalysed reaction by measuring the volume of gas produced as the reaction proceeds. I will use the initial rates of a series of experiments to try and find the orders of reaction with respect to enzyme and substrate. The enzyme in this reaction will be catalase, and the substrate will be hydrogen peroxide (H O ). Catalase is an enzyme and so catalyses the decomposition of hydrogen peroxide, thus producing water and oxygen. This...
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