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T1 coursework effect of substrate concentration on the activity of catalase  

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Aim: This is an experiment to investigate how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase, celery extract. Water displacement and oxygen production will be measured in order to conclude the effect of H2O2 concentration on catalase. The formula: Hydrogen peroxide is a decomposition reaction. This means that the hydrogen peroxide, if left for long enough, will eventually decompose. Hydrogen peroxide Celery Catalase Hydrogen + Oxygen 2H2O2›2H2O + O2 (Aq) (L) (G) Hypothesis As the substrate concentration increases(hydrogen peroxide), the rate of reaction will increase at a directionally proportional rate, until all the hydrogen peroxide has been converted in water and oxygen by the catalase (celery extract). Null Hypothesis. As stated in the hypothesis; "as the substrate concentration increases, the rate of reaction will increase at a directionally proportional rate until the hydrogen peroxide is all converted into water and oxygen and the rate of reaction starts to...

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