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Investigate how the Substrate Hydrogen Peroxide Affects the Rate of an Enzyme Catalysed Reaction. Aim: To investigate how changing the concentration of hydrogen peroxide affects the rate of decomposition. A yeast solution will be used to break down the hydrogen peroxide. Measure the rate of decomposition by recording the amount of oxygen collected in a certain amount of time. Background Knowledge: Hydrogen peroxide is a by-product of metabolism in living cells. It is extremely dangerous for organisms to carry in the body as it is toxic, and therefore must be broken down very quickly. In human cells, the enzyme catalase is produced in the liver, which breaks down hydrogen peroxide into water and oxygen. Hydrogen peroxide Water + Oxygen H2O2 2H2O + O2 The two react because of the "lock and key" theory. The enzyme has a particular shape on its active site, onto which the substrate molecule can fit exactly. The substrate is like a key, into...

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