Influence of pH on the Activity of Potato Catalase.
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| Submitted: Mon Mar 08 2004
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Influence of pH on the Activity of Potato Catalase Catalase is found in a vide variety of plant and animal tissues, its use is to break down toxic hydrogen peroxide which is formed as a by-product of biochemical reactions, into the harmless substances, water and oxygen. The rate of activity of many enzymes is largely influenced by changes in the pH level. To show the effect of pH on the enzyme Catalase, an experiment will be conducted where potato-discs of a constant size are placed in solutions of known pH and will react with hydrogen peroxide in the solution. The rate at which oxygen is evolved will be measured using a manometer, thus reflecting the activity of the Catalase in the potato. Requirements for Experiment: - Scalpel/Sharp Cutting Device - Cork Borer - Petri Dish - Boiling Tube (with rubber bung) - Stand (with bosses and clamps) - Manometer Tube (with a select diameter 2mm/3mm) - Beaker - Syringe (5cm³) X2 -...

