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Effect of an enzyme concentration on the rate of reaction of gelatine.
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Effect of an enzyme concentration on the rate of reaction of gelatine. Aim The aim of the investigation is to find the effect of Trypsin concentration on the rate of digestion. This will be carried out on a gelatine substrate from photographic film. Background Enzymes have an active site which is shaped so that only a molecule, known as the substrate, with the correct shape can link into the enzyme. Once the enzyme and the substrate become associated, the enzyme can increase the probability of a chemical reaction occurring. As the substrate molecule comes into contact with the enzyme's active site it forms a temporary union with the enzyme. Thus, instead of a one-step reaction taking place, enzyme mediated reactions occur via an intermediate step in which the substrate first forms an enzyme-substrate complex which in turn undergoes a second reaction to form the final product and return the enzyme to its original...
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