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Devise an experiment which will give greater understanding about enzyme catalysed reactions
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Investigating Enzyme Substrate Reactions Aim: To devise an experiment which will give greater understanding about enzyme catalysed reactions. Background Information Hydrogen peroxide (H2O2) is a powerful oxidant; it readily breaks down into water and oxygen. The breakdown can be speeded up by introducing the enzyme, oxidoreductase (commonly known as catalase). I will investigate the effect of varying hydrogen peroxide concentrations on the rate of reaction. Prediction I believe that the rate of reaction will increase as the concentration of hydrogen peroxide used increases, I do however think that the rate of reaction will reach a maximum where the active sites of all the enzymes will be saturated. As the concentration of the substrate increases, the chance of collision with the enzyme increases. This will speed up the rate of reaction. It is difficult to estimate the concentration at which the active sites will become saturated. Variables The rate of reaction could be affected by various factors. pH will be kept...
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