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An investigation of a factor affecting the activity of Liver peroxidase enzyme  

Member rating: 2 out of 10 stars (1 vote) | Words: | Submitted: Thu Jan 13 2005

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An investigation of a factor affecting the activity of Liver peroxidase enzyme Apparatus Water bath, a piece of liver (0.2g)- liver peroxidase, 16 boiling tubes, delivery tube, rubber bung, hydrogen peroxide (H2O2), water, sieve, apron, rubber gloves, 100ml measuring cylinder, 25ml measuring cylinder, 10ml measuring cylinder, forceps, watch glass, electronic balance, pipette, scalpel, safety goggles, stop watch, distilled water, boiling tube rack, water proof marker pen, 100ml beaker, clamp, boss, retort-stand. Diagram Method Safety goggles, an apron and rubber gloves were worn through out the whole experiment. First the water bath temperature was turned up to 35º C. While the water was warming up, 0.2g of liver was being cut and weighed on the electronic balance. It was the placed on a watch glass. Then 8 boiling tubes were placed in a boiling tube rack. 19 ml of hydrogen peroxide was measured in a 25 ml measuring cylinder. 1 ml of distilled water...

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